Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome. By Jay Shendure, Gregory J. Porreca, Nikos B. Reppas, Xiaoxia Lin. Request PDF on ResearchGate | Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome | We describe a DNA sequencing technology in which . We apply this technology to resequence an evolved strain of Escherichia coli at less Accurate multiplex polony sequencing of an evolved bacterial genome.
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FPrime is an expert-curated resource to help you find the articles of greatest interest and relevance to you. Defining heterogeneity within bacterial populations via single cell approaches. A cell-free, mate-paired library provided single DNA molecules that were amplified in multiplec to aequencing beads by emulsion polymerase chain reaction.
Accurate multiplex polony sequencing of an evolved bacterial genome.
Reppas and Xiaoxia Lin and John P. Church Published in Science We describe a DNA sequencing technology in which a commonly available, inexpensive epifluorescence microscope is converted to rapid nonelectrophoretic DNA sequencing automation.
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This paper has highly influenced 23 other papers. Cost per base was roughly one-ninth as much as that of conventional sequencing. Rosenbaum and Michael D. Skip to search form Skip to main content. You hereby agree to indemnify and keep indemnified F, its affiliates, contractors and agents from and against any and all losses including without limitation direct, indirect and consequential losscosts, claims, damages or expenses of whatever nature and howsoever caused arising directly or indirectly from any breach of these Terms and Conditions or arising from the Material posted on this website or content contained in any email sent using the facilities provided by the website by you including without limitation as a result of any infringement of any intellectual property or other proprietary rights, libel, defamation, obscenity or the Material being otherwise unlawful.
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Please disclose any competing interests that might be construed to influence your judgment of the validity or importance of the article, or any recommendation or review. Millions of beads were immobilized in a polyacrylamide gel and subjected to polonu cycles of sequencing by ligation and four-color imaging.
The sequence of sequencers: Bacteriql glimpse into past, present, and future DNA sequencing. Bwcterial work at the same institute as any of the authors.
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Topics Discussed in This Paper. We apply this technology to resequence an evolved strain of Escherichia coli at less than one error per million consensus bases. You are a close professional associate of any of the authors e. Citations Publications citing this paper.
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Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome
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